MEK1 anticorps (pSer298) (PE)

N° du produit ABIN1177090

Cet anticorps Souris Monoclonal détecte spécifiquement MEK1 dans ICS. Il présente une réactivité envers Humain et Souris et a été mentionné dans 3+ publications.

Aperçu rapide pour MEK1 anticorps (pSer298) (PE) (ABIN1177090)

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MEK1 est conjugé à/à la PE

Application: Intracellular Staining (ICS)

Clone: J114-64

Détail du produit anti-MEK1 anticorps (PE)

Épitope: pSer298

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human MEK1 Peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: Either BD Cytofix™ buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-MEK1 (PE) (ABIN1177090)

Slack-Davis, Eblen, Zecevic, Boerner, Tarcsafalvi, Diaz, Marshall, Weber, Parsons, Catling: "PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation." dans: The Journal of cell biology, Vol. 162, Issue 2, pp. 281-91, (2003) (PubMed).

Eblen, Slack, Weber, Catling: "Rac-PAK signaling stimulates extracellular signal-regulated kinase (ERK) activation by regulating formation of MEK1-ERK complexes." dans: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6023-33, (2002) (PubMed).

Kolch: "Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions." dans: The Biochemical journal, Vol. 351 Pt 2, Issue 2, pp. 289-305, (2001) (PubMed).

Détails sur MEK1

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Autre désignation: MEK1

Sujet: MEK (Map/Erk Kinase) 1 and 2 are serine/threonine kinases, also known as MAP kinase kinases (MAP2K1 and 2, MAPKK1 and 2, or MKK1 and 2). They activate the MAP (Mitogen-Activated Protein) kinases, also known as ERKs (Extracellular signal Regulated Kinases), which are critical kinases in multiple signal transduction pathways that regulate cell growth and differentiation. Activation of MEK 1 and 2 is dependent upon phosphorylation of serines 218 and/or 222 by activated MAP kinase kinase kinases (MAP3Ks), such as the Raf isoforms. Hormones, growth and differentiating factors, or tumor promoters induce Raf activation via activation of Ras proteins. Alternatively, cellular adhesion can lead to phosphorylation of MEK1 at serine 298 (S298), mediated by p21-activated kinase (PAK). The S298-phosphorylated MEK1 has an enhanced capacity to interact with Raf, resulting in MEK1 activation. The J114-64 monoclonal antibody recognizes the phosphorylated S298 of MEK1.

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling